Introduction to single-cell technologies

Duration: 60 min

Lecture material:

Outline:

  • Introduction: what are single-cell technologies?

  • Single-cell data generation:

    • Sample preparation methods: single-cell isolation

    • Library preparation and sequencing methods

      • 3'/5' end tagging methods: 10X Chromium

      • Full-length methods: Smart-seq2

      • Long-read sequencing methods

  • 3’/5' end vs full-length data:

    • Transcript coverage

    • Sensitivity, sequencing depth, capture efficiency

    • Precision and accuracy for quantification

    • Cell throughput and power

    • Use-cases

  • Multimodal single-cell data

References:

Paper references used throughout the preparation of this lecture are included in the corresponding slides.